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Functional Assessment of Intestinal Permeability and Neutrophil Transepithelial Migration in Mice using a Standardized Intestinal Loop Model
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Chapters
- 00:04Introduction
- 01:29Generation of the Ileal Loop
- 04:29Generation of the Proximal Colon Loop (pcLoop)
- 06:37Results: JAM-A Regulates Intestinal Permeability in vivo and Promotes LTB4-dependent Recruitment of PMN into the Lumen of the pcLoop
- 08:46Conclusion
Dysregulated intestinal epithelial barrier function and immune responses are hallmarks of inflammatory bowel disease that remain poorly investigated due to a lack of physiological models. Here, we describe a mouse intestinal loop model that employs a well-vascularized and exteriorized bowel segment to study mucosal permeability and leukocyte recruitment in vivo.
