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Journal / Immunology and Infection / Modello di co-coltura senza contatto per lo…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Modello di co-coltura senza contatto per lo studio dell'attivazione delle cellule immunitarie innate durante l'infezione da virus respiratorio
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Modello di co-coltura senza contatto per lo studio dell'attivazione delle cellule immunitarie innate durante l'infezione da virus respiratorio

Article DOI: 10.3791/62115-v • 07:36 min • February 28th, 2021
February 28th, 2021 •
*1, *1, 1, 2, 3, 1,4, 1,4, 5, 2,6, 1,2,6, 1,6
1Department of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, 2Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 3Haartman Institute, Medicum, University of Helsinki, 4Department of Otolaryngology, Head and Neck Surgery, National University Hospital, National University Health System, 5Agency for Science, Technology and Research (A*STAR), Singapore Immunology Network (SIgN), 6NUHS Infectious Diseases Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore
* These authors contributed equally

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Questo protocollo descrive in dettaglio un'indagine sulle prime interazioni tra cellule epiteliali nasali infettate viralmente e attivazione cellulare innata. I singoli sottoinsiemi di cellule immunitarie possono essere distinti in base alla loro attivazione in risposta alle infezioni virali. Possono quindi essere ulteriormente studiati per determinare i loro effetti sulle prime risposte antivirali.

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Immunologia e infezione Numero 168 Co-coltura cellule epiteliali nasali umane cellule immunitarie innate cellule T innate virus respiratorio influenza citochine citometria a flusso
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