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Immunology and Infection
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Assessing Biofilm Dispersal in Murine Wounds
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Immunology and Infection
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JoVE Journal Immunology and Infection
Assessing Biofilm Dispersal in Murine Wounds

Assessing Biofilm Dispersal in Murine Wounds

DOI:
10.3791/62136-v

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12:18 min

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August 07, 2021

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, ,
1Department of Surgery,Texas Tech University Health Sciences Center, 2Immunology and Molecular Microbiology,Texas Tech University Health Sciences Center, 3TTUHSC Surgery Burn Center of Research Excellence,Texas Tech University Health Sciences Center

Chapters

  • 00:00Introduction
  • 01:24Dorsal Full Thickness Excision Surgery
  • 03:25In vivo Treatment and Assessment of Dispersal
  • 07:04Ex vivo Assessment of Dispersal
  • 09:12Results: Enzymatic Dispersal Leads to Bacterial Dissemination in the Host
  • 10:55Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe ex vivo and in vivo methods for assessing bacterial dispersal from a wound infection in mice. This protocol can be utilized to test the efficacy of topical antimicrobial and anti-biofilm therapies, or to assess the dispersal capacity of different bacterial strains or species.

Tags

Keywords: BiofilmBiofilm DispersalMurine Wound ModelChronic InfectionBacterial InfectionDispersal AgentAnesthesiaWound ExcisionWound InfectionCFUBacterial Inoculation

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