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Biochemistry

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JoVE Journal Biochemistry
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Platform Incubator with Movable XY Stage: A New Platform for Implementing In-Cell Fast Photochemical Oxidation of Proteins

Article DOI: 10.3791/62153-v • 05:50 min • May 17th, 2021
May 17th, 2021 •
1, 2, 1, 3, 1
1Department of Pharmaceutical Sciences, University of Maryland Baltimore, 2Technology Research Center, University of Maryland Baltimore County, 3Department of Biochemistry and Molecular Biology, University of Massachusetts

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Summary
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A new static platform is used to characterize protein structure and interaction sites in the native cell environment utilizing a protein footprinting technique called in-cell fast photochemical oxidation of proteins (IC-FPOP).

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Platform Incubator Movable XY Stage In-Cell Fast Photochemical Oxidation Of Proteins Biological Questions In-cell FPOP Experiment Protein-ligand Interactions Protein-protein Interaction Sites Conformational Change Automated Six-well Plate-based IC-FPOP Platform Pharmaceutical Industry Biotech Industry Academic Research Institutes Clinical Research Institutes Protein Dynamics Platform Incubator Positioning Stage Temperature Control Gas Control Humidifier Control Cell Culture Hood Six-well Plate Cell Confluency Microscope Tygon Tubes 3D Printed Rings Integration Software Script Peristaltic Pump
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