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Biology

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Chemical Dimerization-Induced Protein Condensates on Telomeres
 

Chemical Dimerization-Induced Protein Condensates on Telomeres

Article DOI: 10.3791/62173-v
April 12th, 2021

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Summary April 12th, 2021

This protocol illustrates a chemically induced protein dimerization system to create condensates on chromatin.  The formation of promyelocytic leukemia (PML) nuclear body on telomeres with chemical dimerizers is demonstrated. Droplet growth, dissolution, localization and composition are monitored with live cell imaging, immunofluorescence (IF) and fluorescence in situ hybridization (FISH).

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