Journal
/
Biology
/
Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
JoVE Journal
Biology
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

DOI:
10.3791/62175-v

•

10:59 min

•

August 21, 2021

•

, , ,
1Institute of Pathology, Faculty of Medicine,University of Szeged

Chapters

  • 00:05Introduction
  • 01:44Immunodetection of Specific Proteins
  • 05:08Chromatin Immunoprecipitation
  • 09:06Representative Results
  • 10:28Conclusion

Summary

Automatic Translation

Automatic Translation

This article introduces essential steps of immunostaining and chromatin immunoprecipitation. These protocols are commonly used to study DNA damage-related cellular processes and to visualize and quantify the recruitment of proteins implicated in DNA repair.

Tags

Keywords: DNA DamageImmunostainingChromatin ImmunoprecipitationDNA RepairLaser MicroirradiationEndonucleaseDouble-strand BreaksNeocarzinostatinU2OS CellsCell CultureFixation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62175?article_type=v

Extracting DNA from the Gut Microbes of the Termite (Zootermopsis Angusticollis) and Visualizing Gut Microbes
check_url/62175?article_type=v

Principles of Site-Specific Recombinase (SSR) Technology
check_url/62175?article_type=v

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
check_url/62175?article_type=v

Imaging Mismatch Repair and Cellular Responses to DNA Damage in Bacillus subtilis
check_url/62175?article_type=v

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
check_url/62175?article_type=v

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts
check_url/62175?article_type=v

Detection and Analysis of DNA Damage in Mouse Skeletal Muscle In Situ Using the TUNEL Method
check_url/62175?article_type=v

In Vivo Alkaline Comet Assay and Enzyme-modified Alkaline Comet Assay for Measuring DNA Strand Breaks and Oxidative DNA Damage in Rat Liver
check_url/62175?article_type=v

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites
check_url/62175?article_type=v

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Read Article