Journal
/
Developmental Biology
/
Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity

Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity

DOI:
10.3791/62182-v

•

10:03 min

•

January 30, 2021

•

, , , , ,
1Department of Pathology, Microbiology and Immunology,University of South Carolina School of Medicine, 2Department of Dermatology,Brigham and Women’s Hospital, Harvard Medical School, 3Department of Biochemistry and Molecular Biology,University of Maryland School of Medicine Baltimore, 4Department of Pathology,Albert Einstein College of Medicine, 5Department of Pediatrics,Vanderbilt University Medical Center, 6Department of Drug Discovery and Biomedical Sciences, College of Pharmacy,University of South Carolina

Chapters

  • 00:04Introduction
  • 00:27Isolation and Culture of Human Keratinocytes from Neonatal Foreskin Tissue
  • 02:37Generating Skin Epidermosphere Cultures in Vitro
  • 05:02Epidermal Spheroid Re-Plating Assay
  • 05:42Characterization of Spheroid-Derived (SD) Sub-Populations by FACS
  • 08:06Results: Characterization of Epidermal Spheroid Formation and Spheroid-Derived NHKs
  • 09:30Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a protocol for the systematic cultivation of epidermal spheroids in 3D suspension culture. This protocol has wide-ranging applications for use in a variety of epithelial tissue types and for the modeling of several human diseases and conditions.

Tags

Keywords: Epidermal SpheroidKeratinocyte Stem CellHigh Throughput CultureNeonatal ForeskinDispaseTrypsin-EDTAKSFM-SCM MediumAgarose96-well Plate

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62182?article_type=v

Forward Genetic Approach to Uncover Stress Resistance Genes in Mice — A High-throughput Screen in ES Cells
check_url/62182?article_type=v

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media
check_url/62182?article_type=v

Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research
check_url/62182?article_type=v

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin
check_url/62182?article_type=v

Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol
check_url/62182?article_type=v

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening
check_url/62182?article_type=v

Evaluation of Injury-induced Senescence and In Vivo Reprogramming in the Skeletal Muscle
check_url/62182?article_type=v

Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution
check_url/62182?article_type=v

A Semi-high-throughput Imaging Method and Data Visualization Toolkit to Analyze C. elegans Embryonic Development
check_url/62182?article_type=v

Isolation, Culture, and Adipogenic Induction of Neural Crest Original Adipose-Derived Stem Cells from Periaortic Adipose Tissue

Read Article