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Developmental Biology

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JoVE Journal Developmental Biology
Estabelecendo um sistema de cultura esferoide epidérmico de alta produtividade para modelar a plasticidade das células-tronco de ceratinócito
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Estabelecendo um sistema de cultura esferoide epidérmico de alta produtividade para modelar a plasticidade das células-tronco de ceratinócito

Article DOI: 10.3791/62182-v • 10:03 min • January 30th, 2021
January 30th, 2021 •
1,2, 3, 4, 5, 6, 1
1Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, 2Department of Dermatology, Brigham and Women's Hospital, Harvard Medical School, 3Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine Baltimore, 4Department of Pathology, Albert Einstein College of Medicine, 5Department of Pediatrics, Vanderbilt University Medical Center, 6Department of Drug Discovery and Biomedical Sciences, College of Pharmacy, University of South Carolina

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Aqui descrevemos um protocolo para o cultivo sistemático de esferoides epidérmicos na cultura de suspensão 3D. Este protocolo possui amplas aplicações para uso em uma variedade de tipos de tecido epitelial e para a modelagem de várias doenças e condições humanas.

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Biologia do Desenvolvimento Questão 167 Anoikis plasticidade celular epitélio células-tronco epidérmicas epidermófera queratinócitos humanos esferoides cultura 3D cicatrização de feridas
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