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Monitoring van neutrofiele Elastase en Cathepsine G-activiteit in menselijke Sputummonsters
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Monitoring Neutrophil Elastase and Cathepsin G Activity in Human Sputum Samples
DOI:

09:23 min

May 21, 2021

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Chapters

  • 00:05Introduction
  • 01:07Cell Isolation and Supernatant Separation
  • 02:36Quantification of Soluble Neutrophil Serine Protease (NSP) Activity via Fluorimeter or Plate Reader Assay
  • 04:13Membrane-bound NSPs Activity Measurement via Fluorescence Microscopy
  • 05:30Membrane-bound NSPs Activity Measurement via Flow Cytometry
  • 07:25Results: Representative Images and Quantification of Membrane-Bound NE Activity on Neutrophils Isolated from CF Patient Sputum
  • 08:26Conclusion

Summary

Automatic Translation

De hierin beschreven protocollen bieden een gids om de activiteit van neutrofiele proteasen in humaan sputum te visualiseren en te kwantificeren. De toepassingen van een dergelijke analyse variëren van de evaluatie van ontstekingsremmende behandelingen tot biomarkervalidatie, geneesmiddelenscreening en grote cohort klinische studies.

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