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JoVE Journal Developmental Biology
Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells
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Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells

Article DOI: 10.3791/62206-v • 05:38 min • February 12th, 2021
February 12th, 2021 •
1,2, 3, 1,2, 1,2,4, 1,2,5, 3, 1,2,5,6
1Center for Cardiovascular Research, The Abigail Wexner Research Institute, Nationwide Children’s Hospital, 2The Heart Center, Nationwide Children’s Hospital, 3Department of Physiology and Cell Biology, The Ohio State University College of Medicine, 4Department of Anatomy, The Ohio State University College of Medicine, 5MCDB Graduate Program, The Ohio State University, 6Department of Pediatrics, The Ohio State University College of Medicine

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Summary
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Here, we present a protocol to robustly generate and expand human cardiomyocytes from patient peripheral blood mononuclear cells.

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Generation Expansion Human Cardiomyocytes Patient Peripheral Blood Mononuclear Cells Protocol Reproducible Easy To Follow Stem Cell Reprogramming Directed Differentiation Cardiovascular Diseases Cardiac Toxicity Novel Drugs IPSC Colonies DPBS EDTA Passaging Medium Basement Membrane Coated Six Well Plate E8 Medium
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