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Immunology and Infection

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JoVE Journal Immunology and Infection
Rening och expansion av Musinvarianta naturliga killer T-celler för in vitro- och in vivo-studier
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Rening och expansion av Musinvarianta naturliga killer T-celler för in vitro- och in vivo-studier

Article DOI: 10.3791/62214-v • 08:37 min • February 15th, 2021
February 15th, 2021 •
1, *1,2, *1,2, 1,2
1Experimental Immunology Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, 2Vita-Salute San Raffaele University
* These authors contributed equally

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Vi beskriver ett snabbt och robust protokoll för att berika invarianta naturliga killer T (iNKT) celler från mus mjälte och expandera dem in vitro till lämpliga nummer för in vitro och in vivo studier.

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Immunologi och infektion nummer 168 iNKT-celler expansion mus Vα14 CD1d anti-CD3/CD28 pärlor aktivering lymfocyter
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