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Large-Scale Preparation of Synovial Fluid Mesenchymal Stem Cell-Derived Exosomes by 3D Bioreactor Culture
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Large-Scale Preparation of Synovial Fluid Mesenchymal Stem Cell-Derived Exosomes by 3D Bioreactor Culture

Large-Scale Preparation of Synovial Fluid Mesenchymal Stem Cell-Derived Exosomes by 3D Bioreactor Culture

DOI:
10.3791/62221-v

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09:48 min

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July 26, 2022

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, , , , , ,
1Department of Orthopedics,The First affiliated hospital of Shenzhen University, Shenzhen Second People’s Hospital, 2Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology,Shenzhen Second People’s Hospital, 3Department of Child and Adolescent Psychiatry, Shenzhen Kangning Hospital, Shenzhen Mental Health Center,Shenzhen Key Laboratory for Psychological Healthcare & Shenzhen Institute of Mental Health

Chapters

  • 00:05Introduction
  • 00:40Human Synovial Fluid Mesenchymal Stem Cells (SF-MSCs) Culture and Identification
  • 02:42Microcarrier Preparation and Perfusion Bioreactor
  • 04:18Exosome Identification and Safety Detection
  • 05:01Nanoparticle Tracking Analysis (NTA) and Western Blotting
  • 06:48Results: Identification and Analysis of SF-MSCs and the Exosomes
  • 08:55Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to produce a large number of GMP-grade exosomes from synovial fluid mesenchymal stem cells using a 3D bioreactor.

Tags

Synovial FluidMesenchymal Stem CellsExosomes3D BioreactorGMP-gradeCell CultureFlow CytometryMicrocarriersCell PreparationCentrifugationCell DensityCell PassageCell PelletCell SuspensionFluorescent AntibodyCell WashingCell Detection

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