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Large-Scale Preparation of Synovial Fluid Mesenchymal Stem Cell-Derived Exosomes by 3D Bioreactor Culture
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Large-Scale Preparation of Synovial Fluid Mesenchymal Stem Cell-Derived Exosomes by 3D Bioreactor Culture

Article DOI: 10.3791/62221-v • 09:48 min • July 26th, 2022
July 26th, 2022 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,3
1Department of Orthopedics, The First affiliated hospital of Shenzhen University, Shenzhen Second People's Hospital, 2Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen Second People's Hospital, 3Department of Child and Adolescent Psychiatry, Shenzhen Kangning Hospital, Shenzhen Mental Health Center, Shenzhen Key Laboratory for Psychological Healthcare & Shenzhen Institute of Mental Health

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Summary
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Here, we present a protocol to produce a large number of GMP-grade exosomes from synovial fluid mesenchymal stem cells using a 3D bioreactor.

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Synovial Fluid Mesenchymal Stem Cells Exosomes 3D Bioreactor Culture GMP-grade Exosomes Exosome Biology Research Clinical Arthritis Treatment Dr. Yujie Liang Centrifugation PBS MSC Culture Medium Cell Density Synovial Fluid Mesenchymal Stem Cells (SF-MSCs) Flow Cytometry
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