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Effektiv dissektion og kultur af primære mus Retinal Pigment Epitel celler

Article DOI: 10.3791/62228-v • 08:33 min • February 10th, 2021
February 10th, 2021 •
1, 1, 1
1Ocular Genomics Institute of Massachusetts Eye and Ear, Harvard Medical School

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Denne protokol, som oprindeligt blev rapporteret af Fernandez-Godino et al. i 20161, beskriver en metode til effektivt at isolere og kultur mus RPE celler, som danner en funktionel og polariseret RPE monolayer inden for en uge på Transwell plader. Proceduren tager cirka 3 timer.

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Biologi Problem 168 Mus RPE primær RPE RPE kulturer RPE isolation okulære lidelser polariseret RPE RPE på Transwells mus øje dissektion
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