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Comparative Analysis of Experimental Methods to Quantify Animal Activity in Caenorhabditis elegans Models of Mitochondrial Disease
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Comparative Analysis of Experimental Methods to Quantify Animal Activity in Caenorhabditis elegans Models of Mitochondrial Disease

Comparative Analysis of Experimental Methods to Quantify Animal Activity in Caenorhabditis elegans Models of Mitochondrial Disease

DOI:
10.3791/62244-v

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05:51 min

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April 04, 2021

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, , , ,
1Mitochondrial Medicine Frontier Program, Division of Human Genetics, Department of Pediatrics,The Children’s Hospital of Philadelphia, 2Department of Pediatrics,University of Pennsylvania Perelman School of Medicine

Chapters

  • 00:04Introduction
  • 00:47Worm Locomotor Activity Analysis in Liquid Medium on Glass Slides
  • 01:37C. elegans Locomotor Activity Recording Analysis in ZebraLab Software
  • 03:30Worm Locomotor Activity Analysis in Liquid Medium in 96-Well Plate Format
  • 04:30Results: Comparative Analysis of Locomotor Activity by ZebraLab and WormScan Software Assays
  • 05:14Conclusion

Summary

Automatic Translation

Automatic Translation

This study presents protocols for two semi-automated locomotor activity analysis approaches in C. elegans complex I disease gas-1(fc21) worms, namely, ZebraLab (a medium-throughput assay) and WormScan (a high-throughput assay) and provide comparative analysis among a wide array of research methods to quantify nematode behavior and integrated neuromuscular function.

Tags

Keywords: Caenorhabditis ElegansMitochondrial DiseaseLocomotor ActivityMotility AssaySemiautomated AssayLiquid MediumZebraLab SoftwareVideo RecordingActivity DetectionWorm BehaviorDrug Screening

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