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An Easy and Flexible Inoculation Method for Accurately Assessing Powdery Mildew-Infection Phenotypes of Arabidopsis and Other Plants
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An Easy and Flexible Inoculation Method for Accurately Assessing Powdery Mildew-Infection Phenotypes of Arabidopsis and Other Plants

An Easy and Flexible Inoculation Method for Accurately Assessing Powdery Mildew-Infection Phenotypes of Arabidopsis and Other Plants

DOI:
10.3791/62287-v

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06:09 min

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March 09, 2021

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, , , , , ,
1Institute for Bioscience and Biotechnology Research,University of Maryland, 2Department of Nutrition and Food Science,University of Maryland College Park, 3Department of Plant Sciences and Landscape Architecture,University of Maryland College Park

Chapters

  • 00:05Introduction
  • 00:42Making a Standard Inoculation Box with a Removable Top Lid Mounted with a Mesh
  • 01:17Making an Inoculation Chamber and Inoculating Plants in Flats
  • 03:13Inoculate Plants with Smaller Inoculation Boxes
  • 03:42Inoculate Detached Leaves in Petri Dishes
  • 04:37Results: Assessment of Inoculation Evenness
  • 05:19Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol for constructing a simple spore-distribution system consisting of an inoculation box with a ~50 µm mesh and a transparent plastic chamber. This can be used to evenly inoculate plants with powdery mildew spores, thereby enabling accurate and reproducible assessment of disease phenotypes of plants under study.

Tags

Keywords: Powdery MildewInoculation MethodArabidopsisPlant DiseaseSpore InoculationMeshInoculation BoxAcrylic SheetPowdery Mildew SporesInfection PhenotypeGrowth ChamberPad4-1 MutantForcepsBlender Brushes

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