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Confocal Laser Scanning Microscopy of Calcium Dynamics in Acute Mouse Pancreatic Tissue Slices
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Biology
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Confocal Laser Scanning Microscopy of Calcium Dynamics in Acute Mouse Pancreatic Tissue Slices

Confocal Laser Scanning Microscopy of Calcium Dynamics in Acute Mouse Pancreatic Tissue Slices

DOI:
10.3791/62293-v

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10:49 min

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April 13, 2021

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, , , , ,
1Institute of Physiology, Faculty of Medicine,University of Maribor, 2Faculty of Natural Sciences and Mathematics,University of Maribor, 3Center for Physiology and Pharmacology,Medical University of Vienna

Chapters

  • 00:05Introduction
  • 01:04Injection of Pancreas with Agarose
  • 02:57Tissue Slice Preparation
  • 05:13Calcium Dye Preparation and Loading
  • 06:22Calcium Imaging
  • 08:17Results: Calcium Dynamics in Acute Mouse Pancreatic Tissue Slices
  • 10:03Conclusion

Summary

Automatic Translation

Automatic Translation

We present the preparation of acute pancreatic tissue slices and their use in confocal laser scanning microscopy to study calcium dynamics simultaneously in a large number of live cells, over long time periods, and with high spatiotemporal resolution.

Tags

Keywords: Confocal Laser Scanning MicroscopyCalcium DynamicsLive Cell Calcium ImagingIntercellular WavesMulticellular Functional ConnectivityEnzymatic And Mechanical Stress ReductionDiabetesBrainPituitaryAgarose InjectionCommon Bile DuctDuodenal PapillaExtracellular SolutionCarbogenEuthanized Adult MouseLaparotomyAgarose Hardening

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