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Bioengineering

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JoVE Journal Bioengineering
Beoordeling van functionele statistieken van de gezondheid van skeletspieren in microtissues van menselijke skeletspieren
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Beoordeling van functionele statistieken van de gezondheid van skeletspieren in microtissues van menselijke skeletspieren

Article DOI: 10.3791/62307-v • 09:30 min • February 18th, 2021
February 18th, 2021 •
1,2, 1,2, 1,2, 1,2,3
1Institute of Biomedical Engineering, University of Toronto, 2Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 3Department of Cell and Systems Biology, University of Toronto

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Dit manuscript beschrijft een gedetailleerd protocol om arrays van 3D menselijke skeletspierenmicrotissues en minimaal invasieve downstream in situ assays van functie te produceren, inclusief contractiele kracht en calciumbehandelingsanalyses.

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Bio-engineering Nummer 168 menselijke skeletspieren elektrische stimulatie calciumbehandeling krachtmeting vereeuwigde myogene voorlopercellen minimaal invasief tissue engineering hoog gehalte driedimensionaal celkweek polydimethylsiloxaan
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