/
/
Real-Time Quantification of Reactive Oxygen Species in Neutrophils Infected with Meningitic Escherichia Coli
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:00Introduction
- 00:50Preparation of the Bacteria Strain
- 02:21Isolation of Neutrophils
- 07:41Measurement of ROS
- 09:51Representative Results
- 12:22Conclusion
Escherichia coli is the leading cause of neonatal Gram-negative bacterial meningitis. During the bacterial infection, reactive oxygen species produced by neutrophils play a major bactericidal role. Here we introduce a method to detect the reactive oxygen species in neutrophils in response to meningitis E. coli.
Tags
Keywords:
Reactive Oxygen SpeciesNeutrophilsEscherichia ColiMeningitisNeonatal Bacterial MeningitisBacteremiaBlood-brain BarrierHost DefenseROS ProductionBacterial ColonyBrain-Heart Infusion MediumRifampicinPeripheral Blood SamplesRed Blood Cell LysisMagnetic Cell SortingWhite Blood CellsNeutrophils
