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Chapters
- 00:04Introduction
- 00:32Filtration Procedure for Seawater Dissolved and Particulate Lipids
- 01:53Liquid-Liquid Extraction of Seawater or Liquid Samples
- 03:23Extraction Protocol for Solids (Modified Folch et al. Extraction)
- 06:17Developing and Conditioning Sequences for Separation of Aquatic Lipid Classes by Rod TLC
- 10:11FAME Derivatization with H2SO4 in MeOH
- 13:17Representative Results
- 14:30Conclusion
This protocol is for the determination of lipids in seawater and biological specimens. Lipids in filtrates are extracted with chloroform or mixtures of chloroform and methanol in the case of solids. Lipid classes are measured by rod thin-layer chromatography with flame ionization detection and their sum gives the total lipid content.
