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1Department of Medicine,University of Colorado Anschutz Medical Campus, 2Medical Scientist Training Program,University of Colorado Anschutz Medical Campus, 3Department of Chemistry and Chemical Biology,Rensselaer Polytechnic Institute, 4Department of Health Sciences,Curtin University, 5Department of Medicine,Denver Health Medical Center
Chapters
- 00:04Introduction
- 00:55Polyacrylamide Gel Electrophoresis of Isolated and Purified Glycosaminoglycans
- 02:48Silver Staining Protocol
- 03:55Results: Sensitivity and Specificity of Method of Detection of GAGs
- 05:27Conclusion
Denne rapport beskriver teknikker til at isolere og rense sulfat glycosaminoglycaner (GAGs) fra biologiske prøver og en polyacrylamidgelelektroforesetilgang for at tilnærme deres størrelse. GAG'er bidrager til vævsstruktur og påvirker signalprocesser via elektrostatisk interaktion med proteiner. GAG polymer længde bidrager til deres bindende affinitet for cognate ligands.
