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Detektion av glykosaminoglykaner av polyakrylamidgelelektrofores och silverfärgning
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Detektion av glykosaminoglykaner av polyakrylamidgelelektrofores och silverfärgning

Article DOI: 10.3791/62319-v • 05:57 min • February 25th, 2021
February 25th, 2021 •
1,2, 3,4, 1, 1, 3, 1,5
1Department of Medicine, University of Colorado Anschutz Medical Campus, 2Medical Scientist Training Program, University of Colorado Anschutz Medical Campus, 3Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, 4Department of Health Sciences, Curtin University, 5Department of Medicine, Denver Health Medical Center

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Denna rapport beskriver tekniker för att isolera och rena sulferade glykosaminoglykaner (GAGs) från biologiska prover och en polyakrylamid gel elektrofores strategi för att approximera deras storlek. GAGs bidrar till vävnadsstruktur och påverkar signalprocesser via elektrostatisk interaktion med proteiner. GAG polymer längd bidrar till deras bindande affinitet för konjak ligands.

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Biologi nummer 168
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