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Journal / Immunology and Infection / Gene Knock-in por CRISPR/Cas9 e Classificação…
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Immunology and Infection
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JoVE Journal Immunology and Infection
Gene Knock-in por CRISPR/Cas9 e Classificação celular em linhas de células macrófagos e t
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Gene Knock-in por CRISPR/Cas9 e Classificação celular em linhas de células macrófagos e t

Article DOI: 10.3791/62328-v • 11:32 min • November 13th, 2021
November 13th, 2021 •
1, 1, 1, 1, 1, 1, 1, 1, 2, 1
1The Laboratory of Genetic Regulators in the Immune System, School of Laboratory Medicine, Xinxiang Medical University, 2Centre d’Immunologie de Marseille-Luminy, Aix Marseille Université

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Este protocolo usa repórteres fluorescentes e classificação celular para simplificar experimentos de knock-in em linhas de células macrófagos e t. Dois plasmídeos são usados para esses experimentos simplificados de knock-in, ou seja, um plasmídeo de expressão CRISPR/Cas9 e DsRed2 e um plasmídeo de recombinação homólogo que expressa EBFP2, que está permanentemente integrado no lócus Rosa26 em células imunes.

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Imunologia e Infecção Problema 177 knock-in CRISPR/Cas9 Rosa26,macrófago célula T hACE2
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