Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Preparing Lamellae from Vitreous Biological…
JoVE Journal
Biochemistry

This content is Free Access.

JoVE Journal Biochemistry
Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography
Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo
Journal (Biochemistry)
Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo
Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases
Journal (Biochemistry)
Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases
 

Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography

Article DOI: 10.3791/62350-v • 07:00 min • August 5th, 2021
August 5th, 2021 •
1,2, 3,4, 3, 1, 1
1Centre for Ultrastructural Imaging, New Hunt’s House, Guy’s Campus, King’s College London, 2Department of Biological Science, Birkbeck College, University of London, 3Electron Bio-Imaging Centre, Diamond Light Source, Harwell Science and Innovation Campus, 4SLAC National Accelerator Laboratory, Stanford University

Chapters

Summary
Automatic Translation

Using focused ion beam milling to produce vitreous on-grid lamellae from plunge frozen biological samples for cryo-electron tomography.

Tags

Preparing Lamellae Vitreous Biological Samples Dual-Beam Scanning Electron Microscope Cryo-Electron Tomography Plasmodium-falciparum-infected Red Blood Cells Structural Information Malaria Parasite Cryo-electron Tomography Protein Complexes Three-dimensional Structural Information Cell Types Frozen Copper Grids CryoStage Light Microscope Ice Gradient Cell Coverage Cryo-FIB-SEM-specific Autogrid Rims Carbon Or Platinum E-beam Rotary Coat Cell Distribution
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter