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Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography
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Biochemistry
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JoVE Journal Biochemistry
Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography

Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography

DOI:
10.3791/62350-v

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07:00 min

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August 05, 2021

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, , , ,
1Centre for Ultrastructural Imaging,New Hunt’s House, Guy’s Campus, King’s College London, 2Department of Biological Science,Birkbeck College, University of London, 3Electron Bio-Imaging Centre,Diamond Light Source, Harwell Science and Innovation Campus, 4SLAC National Accelerator Laboratory,Stanford University

Chapters

  • 00:04Introduction
  • 00:48Screening Plunge Frozen Grids and Preparing for FIB-Milling
  • 01:28On-Grid FIB Milling of Plunge Frozen Cells
  • 04:18Results: Analysis of Cryo-ET Data from FIB-Milled Lamella
  • 06:28Conclusion

Summary

Automatic Translation

Automatic Translation

Using focused ion beam milling to produce vitreous on-grid lamellae from plunge frozen biological samples for cryo-electron tomography.

Tags

Keywords: Vitreous LamellaeCryo-electron TomographyPlasmodium FalciparumRed Blood CellsMalariaProtein ComplexesCryo-FIB-SEMOrganoplatinum CoatingIon Beam Milling

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