Generation of a Simplified Three-Dimensional Skin-on-a-chip Model in a Micromachined Microfluidic Platform

Ignacio Risue&#241;o; Leticia Valencia; Miguel Holgado; Jose L. Jorcano; Diego Velasco

doi:10.3791/62353

Journal

/

Bioengineering

/

Generation of a Simplified Three-Dimensional Skin-on-a-chip Model in a Micromachined Microfluidic Platform

JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Bioengineering

Generation of a Simplified Three-Dimensional Skin-on-a-chip Model in a Micromachined Microfluidic Platform

4,126 Views

•

06:30 min

•

May 17, 2021

DOI:

10.3791/62353-v

DOI:

10.3791/62353-v

•

06:30 min

May 17, 2021

•

4072 Views

Ignacio Risueño¹, Leticia Valencia¹, Miguel Holgado², Jose L. Jorcano³, Diego Velasco^1,4

¹Department of Bioengineering and Aerospace Engineering,Universidad Carlos III de Madrid (UC3M)²Group of Optics, Photonics and Biophotonics (GOFB). Center for Biomedical Technology,Universidad Politécnica de Madrid ³Division of Epithelial Biomedicine,CIEMAT ⁴Instituto de Investigación Sanitaria Gregorio Marañón

Summary

Here, we present a protocol to generate a three-dimensional simplified and undifferentiated skin model using a micromachined microfluidic platform. A parallel flow approach allows the in situ deposition of a dermal compartment for the seeding of epithelial cells on top, all controlled by syringe pumps.