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Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer
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Chapters
- 00:04Introduction
- 01:00Heated Fixture Preparation
- 02:51Characterization of Temperature-Dependent Behaviors in DIBs
- 07:33Results: Characterization of a Droplet Interface Bilayer Formed by Temperature Controlled Assembly
- 09:07Conclusion
This protocol details the use of a feedback temperature-controlled heating system to promote lipid monolayer assembly and droplet interface bilayer formation for lipids with elevated melting temperatures, and capacitance measurements to characterize temperature-driven changes in the membrane.
Tags
Keywords: Droplet Interface BilayerDIBTemperature-controlled AssemblyCharacterizationMembraneHeat TransferThermocoupleAluminum FixtureAcrylic ReservoirHexadecane OilInsulative RubberResistive Heating ElementsElectrical/optical CharacterizationTemperature-related EffectsCellular EnvironmentsTransport And Signaling PropertiesMembrane ConstituentsNatural Cellular Extracts
