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Dissekera mekanoenzymatiska egenskaper hos processiva myosiner med ultrasnabb kraftklämspektroskopi
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Dissekera mekanoenzymatiska egenskaper hos processiva myosiner med ultrasnabb kraftklämspektroskopi

Article DOI: 10.3791/62388-v • 09:38 min • July 1st, 2021
July 1st, 2021 •
1,2, 2,3, 1,2,3, 2,3
1National Institute of Optics, National Research Council, 2LENS, European Laboratory for Non-Linear Spectroscopy, 3Physics Department, University of Florence

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Här presenteras ett omfattande protokoll för att utföra ultrasnabba kraftklämma experiment på processiva myosin-5-motorer, som lätt kan utvidgas till studier av andra klasser av processiva motorer. Protokollet beskriver alla nödvändiga steg, från installationen av experimentapparaten till provberedning, datainsamling och analys.

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Biologi nummer 173 ultrasnabb kraftklämspektroskopi optisk pincett tre-pärlanalys molekylära motorer myosin
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