/
/
Automated Dissection Protocol for Tumor Enrichment in Low Tumor Content Tissues
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Departments of Research Pathology,Genentech, 2Bioinformatics & Computational Biology,Genentech, 3Roche Sequencing Solutions, Hacienda Drive, 4Department of Medicine,Vanderbilt University Medical Center, Medical Center Drive, 5Department of Pathology,Northwestern University, Feinberg School of Medicine
Chapters
- 00:05Introduction
- 00:43Automated Tissue Dissection
- 05:23Results: Confirmation of the Automated Dissection
- 06:19Conclusion
Digital annotation with automated tissue dissection provides an innovative approach to enriching tumor in low tumor content cases and is adaptable to both paraffin and frozen tissue types. The described workflow improves accuracy, reproducibility and throughput and could be applied to both research and clinical settings.
