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JoVE Journal Genetics
Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
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Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae

Article DOI: 10.3791/62423-v • 09:25 min • June 2nd, 2021
June 2nd, 2021 •
*1, *1, 1, 1, 1, 1, 1
1Beijing Key Laboratory of New Technology in Agricultural Application, National Demonstration Center for Experimental Plant Production Education, Department of Agronomy, Beijing University of Agriculture
* These authors contributed equally

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Summary
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Here, we present a protocol to analyze the genome-wide distribution of histone modifications, which can identify new target genes in the pathogenesis of M. oryzae and other filamentous fungi.

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Genome-wide Analysis Histone Modifications Chromatin Immunoprecipitation Sequencing Magnaporthe Oryzae Epigenetic Modifications Fungal Pathogenesis Plant Pathology DNA Segments Histones Transcription Factors Efficiency And Resolution Liquid Complete Medium Oatmeal Tomato Agger Plates Inoculation Loop Wild Type Strain Knockout Strain Mycelia Debris Sodium Chloride Solution Lysis Enzyme Permeation Solution Fungal Hyphae Licing At 28 Degrees Celsius Shaking At 150 RPM Protoplast Formaldehyde
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