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Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice
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JoVE Journal Cancer Research
Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice

Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice

DOI:
10.3791/62442-v

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07:36 min

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June 12, 2021

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, , , , , , , , , , , , , , , , , ,
1Institute of Immunology,Third Military Medical University, 2Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Stomatological Hospital,Sun Yat-Sen University, 3Shigatse Branch, Xinqiao Hospital,Third Military Medical University, 4Department of Emergency Medicine, Southwest Hospital,Third Military Medical University, 5Cancer Center,The General Hospital of Western Theater Command

Chapters

  • 00:04Introduction
  • 00:56Ectopic Tumor Implantation of B16F10-OVA Cells in the Inguinal Region of Mice
  • 02:23Adoptive Transfer of Congenically Marked OT-I T Cells into Tumor-Bearing Mice
  • 03:26Dissect Tumor Mass from Tumor-Bearing Donor Mice
  • 04:31Subcutaneous Transplantation of Donor-Derived Tumor onto the Tumor-Matched Recipient Mice
  • 06:14Results: For Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice
  • 07:04Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a tumor transplantation protocol for the characterization of tumor-inherent and periphery-derived tumor-infiltrated lymphocytes in a mouse tumor model. Specific tracing of the influx of recipient-derived immune cells with flow cytometry reveals the dynamics of the phenotypic and functional changes of these cells during antitumor immune responses.

Tags

Keywords: Tumor TransplantationTumor-infiltrating CD8+ T CellsTumor ImmunologyB16F10-OVAOT-1 CellsTumor Size MeasurementTail Vein Injection

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