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JoVE Journal Cancer Research
Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice
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Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice

Article DOI: 10.3791/62442-v • 07:36 min • June 12th, 2021
June 12th, 2021 •
1, 1, 2, 1, 2, 1, 3, 1, 1, 1, 1, 1, 1, 1, 1, 4, 1, 5, 1
1Institute of Immunology, Third Military Medical University, 2Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Stomatological Hospital, Sun Yat-Sen University, 3Shigatse Branch, Xinqiao Hospital, Third Military Medical University, 4Department of Emergency Medicine, Southwest Hospital, Third Military Medical University, 5Cancer Center, The General Hospital of Western Theater Command

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Summary
Automatic Translation

Here, we present a tumor transplantation protocol for the characterization of tumor-inherent and periphery-derived tumor-infiltrated lymphocytes in a mouse tumor model. Specific tracing of the influx of recipient-derived immune cells with flow cytometry reveals the dynamics of the phenotypic and functional changes of these cells during antitumor immune responses.

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Tumor Transplantation Tumor-infiltrating CD8+ T Cells Tumor Immunology Peripheral Tissues Tumor Microenvironment Immunotherapy Dynamic Changes Phenotypic Changes Functional Changes B16F10-OVA Cell Suspension Tuberculin Syringe Restrain The Mouse Left Inguinal Region Electric Shaver 75% Ethanol Needle Insertion Subcutaneous Tissue
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