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Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells

Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells

DOI:
10.3791/62448-v

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12:38 min

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November 06, 2021

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, ,
1Department of Chemical and Materials Engineering, Donadeo Innovation Centre for Engineering,University of Alberta, 2School of Life Science and Engineering,Southwest Jiaotong University

Chapters

  • 00:03Introduction
  • 02:08Design and Development of Peptide Library
  • 02:56In vitro Cell Culture
  • 04:42Fura-2 Calcium Assay
  • 05:35Cell Activation and Fluorescent Reading
  • 09:28Data Analysis
  • 09:38Representative Results
  • 12:13Conclusion

Summary

Automatic Translation

Automatic Translation

Techniques for generating a library of short peptides that can activate mast cells via the MRGPRX2 receptor are described. Associated techniques are easy, inexpensive, and can be extended to other cell receptors.

Tags

Keywords: MRGPRX2Mast CellsPeptide LibraryAlanine ScanningN-TruncatedC-TruncatedN+C-TruncatedSolid Phase Peptide SynthesisFura-2 AM Calcium Sensitive DyeGrynkiewicz EquationCalcium Signaling

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