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JoVE Journal Developmental Biology
Un metodo semplificato per la generazione di organoidi renali da cellule staminali pluripotenti umane
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Un metodo semplificato per la generazione di organoidi renali da cellule staminali pluripotenti umane

Article DOI: 10.3791/62452-v • 07:39 min • April 13th, 2021
April 13th, 2021 •
1, 1, 2, 2, 2, 1,3
1Department of Developmental Biology, University of Pittsburgh, School of Medicine, 2Department of Molecular Medicine and Pathology, School of Medical Sciences, University of Auckland, 3Center for Critical Care Nephrology, University of Pittsburgh, School of Medicine

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Qui descriviamo un protocollo per generare organoidi renali da cellule staminali pluripotenti umane (hPSC). Questo protocollo genera organoidi renali entro due settimane. Gli organoidi renali risultanti possono essere coltivati in fiaschi spinner su larga scala o piastre magnetiche multi-pozzo per approcci paralleli di test antidroga.

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Biologia dello sviluppo Numero 170 organoidi renali CHIR99021 sostituzione del siero knock-out corpi embrioidi tessuto renale cellule staminali pluripotenti coltura in sospensione
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