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Control of Cell Geometry through Infrared Laser Assisted Micropatterning
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Bioengineering
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JoVE Journal Bioengineering
Control of Cell Geometry through Infrared Laser Assisted Micropatterning

Control of Cell Geometry through Infrared Laser Assisted Micropatterning

DOI:
10.3791/62492-v

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11:04 min

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July 10, 2021

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, , ,
1Department of Cell and Systems Biology,University of Toronto

Chapters

  • 00:00Introduction
  • 01:16Coating Coverslips with PVA
  • 02:09Setting Up Optical Configurations
  • 06:47Mounting the Coverslip and Setting Up the Macro
  • 08:45Results
  • 10:15Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol presented here enables automated fabrication of micropatterns that standardizes cell shape to study cytoskeletal structures within mammalian cells. This user-friendly technique can be set up with commercially available imaging systems and does not require specialized equipment inaccessible to standard cell biology laboratories.

Tags

Keywords: MicropatterningCell GeometryInfrared LaserAutomated WorkflowImage ProcessingProtein DistributionPVA SolutionCoverslip SpinnerMultiphoton Imaging SystemLaser-assisted PatterningCell ShapeIntercellular MachinesAutomated Micropatterning

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