The protocol presented here enables automated fabrication of micropatterns that standardizes cell shape to study cytoskeletal structures within mammalian cells. This user-friendly technique can be set up with commercially available imaging systems and does not require specialized equipment inaccessible to standard cell biology laboratories.
Yang, S., Tuo, C., Iu, E., Plotnikov, S. V. Control of Cell Geometry through Infrared Laser Assisted Micropatterning. J. Vis. Exp. (173), e62492, doi:10.3791/62492 (2021).