<em>In vitro</em> Induction of Human Dental Pulp Stem Cells Toward Pancreatic Lineages

Suryo Kuncorojakti; Watchareewan Rodprasert; Quynh Dang Le; Thanaphum Osathanon; Prasit Pavasant; Chenphop Sawangmake

doi:10.3791/62497

In vitro Induction of Human Dental Pulp Stem Cells Toward Pancreatic Lineages

JoVE Journal
Bioengineering
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JoVE Journal Bioengineering

In vitro Induction of Human Dental Pulp Stem Cells Toward Pancreatic Lineages

In vitro Induction of Human Dental Pulp Stem Cells Toward Pancreatic Lineages

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07:32 min

September 25, 2021

DOI: 10.3791/62497-v

Suryo Kuncorojakti*^1,2, Watchareewan Rodprasert*¹, Quynh Dang Le^1,5, Thanaphum Osathanon^3,4, Prasit Pavasant^3,4, Chenphop Sawangmake^1,5,6

¹Veterinary Stem Cell and Bioengineering Innovation Center (VSCBIC), Veterinary Pharmacology and Stem Cell Research Laboratory, Faculty of Veterinary Science,Chulalongkorn University, ²Department of Veterinary Anatomy, Faculty of Veterinary Medicine,Universitas Airlangga, ³Department of Anatomy, Faculty of Dentistry,Chulalongkorn University, ⁴Center of Excellence in Regenerative Dentistry, Faculty of Dentistry,Chulalongkorn University, ⁵Veterinary Stem Cell and Bioengineering Research Unit, Faculty of Veterinary Science,Chulalongkorn University, ⁶Department of Pharmacology, Faculty of Veterinary Science,Chulalongkorn University

This protocol presents a comparison between two different induction protocols for differentiating human dental pulp stem cells (hDPSCs) toward pancreatic lineages in vitro: the integrative protocol and the non-integrative protocol. The integrative protocol generates more insulin producing cells (IPCs).

This video is showing the in vitro induction of human dental pulp stem cells toward pancreatic lineages. Stem cells give an alternative treatment in many diseases including diabetes type I which cause biolaging of beta cells. Transplantation of insulin producing cells will be the new promising method for causing lifelong.

In this video two approaches for generating insulin producing cell, including the integrative and non integrative induction protocol are described. And in this part the integration protocol for inducing insulin producing cells from human dental pulp stem cell is explained. For the induction protocol is the for mixing the the natural pancreatic developmental process.

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Human Dental Pulp Stem Cells In Vitro Induction Pancreatic Lineages Insulin Producing Cells Diabetes Type I Integrative Protocol Non-integrative Protocol PDX-1 Overexpression Mesenchymal Stem Cells Differentiation Potential Transduction Transplantation Three Step Induction Approach Ethical Approval