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Cancer Research

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JoVE Journal Cancer Research
Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles
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Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles

Article DOI: 10.3791/62504-v • 08:02 min • May 14th, 2021
May 14th, 2021 •
*1, *2, 2, 2, 3, 2, 1,2
1Istituti Clinici Scientifici Maugeri IRCCS, 2Dipartimento di Scienze Biomediche e Cliniche “L. Sacco”, Università di Milano, 3Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca
* These authors contributed equally

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Summary
Automatic Translation

This paper aims to provide a protocol for the isolation and culture of primary cancer-associated fibroblasts from a syngeneic murine model of triple-negative breast cancer and their application for the preclinical study of novel nanoparticles designed to target the tumor microenvironment. 

Tags

Isolation Primary Cancer-associated Fibroblasts Murine Model Breast Cancer Targeted Nanoparticles Protocol Tumor Microenvironment In Vitro Studies Tumor Stroma Digestion Mix Dissociation Program Mechanical Dissociater Incubate Shaking Cell Strainer RPMI 1640 Medium Centrifuge
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