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Journal / Biochemistry / Simple In-House Ultra-High Performance Capillary…
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Biochemistry
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JoVE Journal Biochemistry
Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
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Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Article DOI: 10.3791/62522-v • 13:36 min • December 4th, 2021
December 4th, 2021 •
1, 1, 2
1Laboratory of Bioinformatics Methods in Combinatorial Chemistry and Biology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 2Department of Molecular Neuroimmune Signalling, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

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Here we present a protocol for the optimized FlashPack capillary column packing procedure. Application of an optimized protocol to a common 100-bar pressure bomb setup allows 10-times faster packing and manufacturing of long ultra-high performance capillary columns.

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In-house Ultra-high Performance Capillary Column FlashPack Approach LC-MS Proteomics Analysis Mass Spectrometry Liquid Chromatography Peptide Separation Internal Diameter Sorbent Column Size Customization Packing Procedure Sorbent Slurry Concentration Pressure Bomb Packing Setup Sorbent Clusters
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