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Staining the Cytoplasmic Ca2+ with Fluo-4/AM in Apple Pulp
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Staining the Cytoplasmic Ca2+ with Fluo-4/AM in Apple Pulp

Article DOI: 10.3791/62526-v • 08:05 min • November 6th, 2021
November 6th, 2021 •
1, 1, 1, 1
1College of Horticulture, Qingdao Agricultural University

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Summary
Automatic Translation

Isolated protoplasts of apple pulp cells were loaded with a calcium fluorescent reagent to detect cytoplasmic Ca2+ concentration.

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Cytoplasmic Ca2+ Fluo-4/AM Staining Apple Pulp Calcium Imaging Small Molecule Fluorescent Indicators Esterification Incubation Loading Solution Protoplast Extraction Enzyme Solution Basic Solution Fluorescence Reagent Staining Fluo-4/AM Loading Solution
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