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Staining the Cytoplasmic Ca2+ with Fluo-4/AM in Apple Pulp
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JoVE Journal Biology
Staining the Cytoplasmic Ca2+ with Fluo-4/AM in Apple Pulp

Staining the Cytoplasmic Ca2+ with Fluo-4/AM in Apple Pulp

DOI:
10.3791/62526-v

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08:05 min

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November 06, 2021

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, , ,
1College of Horticulture,Qingdao Agricultural University

Chapters

  • 00:03Introduction
  • 00:57Protoplast Extraction
  • 01:49Small-molecule Calcium Ion Fluorescence Staining
  • 04:46Protoplast Viability Assay
  • 05:48Results
  • 07:21Conclusion

Summary

Automatic Translation

Automatic Translation

Isolated protoplasts of apple pulp cells were loaded with a calcium fluorescent reagent to detect cytoplasmic Ca2+ concentration.

Tags

Cytoplasmic Ca2+Fluo-4/AMApple PulpCalcium ImagingProtoplast ExtractionSmall Molecule Fluorescent IndicatorCalcium Ion SelectivityEsterification IncubationEnzymatic SolutionFluorescence Microscope

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