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JoVE Journal Genetics
Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
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Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Article DOI: 10.3791/62583-v • 06:59 min • March 31st, 2022
March 31st, 2022 •
*1, *2, 1, 1, 2,3
1Division of Biology, Kansas State University, 2Kansas State Veterinary Diagnostic Laboratory, Kansas State University, 3Department of Diagnostic Medicine/Pathobiology, Kansas State University
* These authors contributed equally

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Summary
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Presented here is sgRNA/CAS9 endonuclease and next-generation sequencing protocol that can be used to identify the mutations associated with double strand break repair near the CD4 promoter.

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Next Generation Sequencing Mutations Repair CAS9-induced Double-strand Break CD4 Promoter Cost-effective Robust Genomic Locus Transfectable Cell Lines Human Keratinocyte Culture Media Growth Supplement Penicillin Streptomycin Trypsin-EDTA FBS-supplemented Media Centrifuge Tube Concentration Of Cells
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