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Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration
JoVE Journal
Biology
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JoVE Journal Biology
Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration
DOI:

06:22 min

July 08, 2021

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Chapters

  • 00:04Introduction
  • 00:43Harvest and Preparation of Intervertebral Disc (IVD) Samples
  • 02:11Tissue Fixation and Histologic Sectioning
  • 03:00Microscopic Imaging and Processing
  • 04:11Cellular Pattern Identification and Density Assessment
  • 04:29Results: Microscopic Analysis of IVDs Through Various Developmental Stages
  • 05:45Conclusion

Summary

Automatic Translation

We present a method to investigate spatial chondrocyte organization in the anulus fibrosus of the intervertebral disc using an optical sectioning method.

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