Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies

6.3K views

Cited by 9

14:56 min

May 6th, 2022

10.3791/62598-v

May 6th, 2022

6.3K views

Natália Yumi Noronha^*¹ , Guilherme da Silva Rodrigues^*¹ , Marcela Augusta de Souza Pinhel¹ , Jean-Baptiste Cazier²^,³ , Lígia Moriguchi Watanabe¹ , Albert Nobre Menezes⁴ , Carlos Roberto Bueno⁵ , Carolina Ferreira Nicoletti¹ , Bruno Affonso Parenti de Oliveira¹ , Isabelle Mello Schineider⁶ , Isabella Harumi Yonehara Noma⁷ , Igor Caetano Dias Alcarás⁸ , Fernando Barbosa⁹ , Carla Barbosa Nonino⁶

¹Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, ²Centre for Computational Biology, University of Birmingham, ³Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, ⁴Cancer Genetics and Evolution Laboratory, Cancer Research UK, Institute of Genetics & Molecular Medicine, The University of Edinburgh, ⁵Ribeirão Preto School of Physical Education and Sport, University of São Paulo, ⁶Health Sciences Department, Ribeirao Preto Medical School, University of Sao Paulo, ⁷Faculty of Pharmaceutical Sciences, University of São Paulo, ⁸Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, ⁹Department of Clinical, Bromatological and Toxicological Analysis, Faculty of Pharmaceutical Sciences of Ribeirao Preto, University of São Paulo

^* These authors contributed equally

The present study describes the workflow to manage DNA methylation data obtained by microarray technologies. The protocol demonstrates steps from sample preparation to data analysis. All procedures are described in detail, and the video shows the significant steps.