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JoVE Journal
Immunology and Infection

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Longitudinal Follow-Up of Urinary Tract Infections and Their Treatment in Mice using Bioluminescence Imaging
 

Longitudinal Follow-Up of Urinary Tract Infections and Their Treatment in Mice using Bioluminescence Imaging

Article DOI: 10.3791/62614-v 07:39 min June 14th, 2021
June 14th, 2021

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Summary

This manuscript describes the intravesical administration of uropathogenic bacteria with a lux operon to induce a urinary tract infection in mice and subsequent longitudinal in vivo analysis of the bacterial load using bioluminescence imaging.

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Tags

Longitudinal Follow-up Urinary Tract Infections Treatment Mice Bioluminescence Imaging Colony Forming Units Reproducibility UTI Field In Vivo Research Bladder Physiology UTI Pathogenesis Susceptibility Real-time Insight Evolution Of Infection Animals Needed Recurrent Infections Chronic Infections Ascending Infections Dissemination To Blood Glycerol Stock Of Bacteria LB Plates Kanamycin Sulfate Culturing Overnight Polystyrene Round Bottom Tube Snap Caps LB Broth Inoculation Loop
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