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Journal / Cancer Research / 인간 말초 혈액 림프구의 γ-H2AX 포시 분석에 대한 자동화된 현미경 채점…
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Cancer Research
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JoVE Journal Cancer Research
인간 말초 혈액 림프구의 γ-H2AX 포시 분석에 대한 자동화된 현미경 채점 방법
Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice
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Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice
Exploring Mitochondrial Energy Metabolism of Single 3D Microtissue Spheroids Using Extracellular Flux Analysis
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인간 말초 혈액 림프구의 γ-H2AX 포시 분석에 대한 자동화된 현미경 채점 방법

Article DOI: 10.3791/62623-v • 08:23 min • December 25th, 2021
December 25th, 2021 •
1, 2, 1, 3, 1, 1, 1, 1, 4, 5, 6, 1
1Radiation Biophysics Division, Nuclear Medicine Department, iThemba LABS, 2Department of Biotechnology, University of the Western Cape, 3Department of Medical Biosciences, University of the Western Cape, 4Division of Medical Physiology, Department of Medicine and Health Sciences, Stellenbosch University, 5Biodosimetry Service, Instituto de Investigaciones Biológicas Clemente Estable and Academic Unit on Radiation Protection, Faculty of Medicine, University of the Republic, 6MetaSystems Hard & Software GmbH

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이 프로토콜은 말초 혈액 림프구에 대한 γ-H2AX 포시 분석의 슬라이드 준비 및 자동 채점을 제공합니다. 분석의 방법 및 민감도를 설명하기 위해, 격리된 림프구는 시험관내에서조사되었다. DNA DSB 검출의 이 자동화된 방법은 빠르고 높은 처리량 생물학 도시법 응용에 유용합니다.

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암 연구 문제 178
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