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In vitro-bedömning av hjärt omprogrammering genom att mäta hjärtspecifikt kalciumflöde med en GCaMP3-reporter
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In vitro-bedömning av hjärt omprogrammering genom att mäta hjärtspecifikt kalciumflöde med en GCaMP3-reporter

Article DOI: 10.3791/62643-v • 05:04 min • February 22nd, 2022
February 22nd, 2022 •
*1,2, *1, 1
1Department of Cardiac Surgery, Cardiovascular Center, The University of Michigan, Ann Arbor, 2Department of Cardiovascular Medicine, Xiangya Hospital, Central South University
* These authors contributed equally

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Vi beskriver här etablering och tillämpning av en Tg (Myh6-cre)1Jmk/J /Gt(ROSA)26Sortm38(CAG-GCaMP3)Hze/J (kallas αMHC-Cre/Rosa26A-Flox-Stop-Flox-GCaMP3 nedan) mus reporter linje för hjärt omprogrammering bedömning. Neonatal hjärtfibblaster (NCFs) isolerade från musstammen omvandlas till inducerade kardiomyocyter (iCMs), vilket möjliggör bekväm och effektiv utvärdering av omprogrammering effektivitet och funktionell mognad av iCMs via kalcium (Ca2 +) flöde.

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Medicin nummer 180
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