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Anterior Segment Organ Culture Platform for Tracking Open Globe Injuries and Therapeutic Performance
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Bioengineering
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JoVE Journal Bioengineering
Anterior Segment Organ Culture Platform for Tracking Open Globe Injuries and Therapeutic Performance

Anterior Segment Organ Culture Platform for Tracking Open Globe Injuries and Therapeutic Performance

DOI:
10.3791/62649-v

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07:27 min

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August 25, 2021

•

,
1Department of Sensory Trauma, United States Army Institute of Surgical Research,Fort Sam Houston, 2Engineering Processes and Product Development Group, United States Army Institute of Surgical Research,Fort Sam Houston

Chapters

  • 00:04Introduction
  • 00:38Dissection Setup
  • 01:07Tissue Dissection
  • 02:15Anterior Segments Setup in Organ Culture Dishes
  • 03:58Starting Anterior Segment Organ Culture
  • 04:30Open Globe Injury Induction
  • 05:29Results: Corneal Integrity and Intraocular Pressure Assessment after Open Globe Injury
  • 06:53Conclusion

Summary

Automatic Translation

Automatic Translation

Open globe eye injuries may go untreated for multiple days in rural or military-relevant scenarios, resulting in blindness. Therapeutics are needed to minimize loss of vision. Here, we detail an organ culture open globe injury model. With this model, potential therapeutics for stabilizing these injuries can be properly evaluated.

Tags

Anterior SegmentOrgan CultureOpen Globe InjuryEyeCorneaIrisTrabecular MeshworkVitreous HumorLensCiliary BodyDissectionCulture PlatformTherapeutic Assessment

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