Three-Dimensional Collagen Matrix Scaffold Implantation as a Liver Regeneration Strategy

This method involves placing an implant of collagen matrix as a scaffold in the liver to promote liver regeneration. This implantation method has opened the door for a strategist exploring the restoration of the shape and balloon of the resected tissues, thereby reducing anesthesia, surgical duration, and recovery time. This method can be used to promote regeneration involving models of liver fibrosis, as well as other mammalian species.

This method is based on tissue engineering and regenerative medicine to promote the restoration of injured tissue. Begin by sanitizing the surgical area work table, microsurgery microscope, and seat with a 2%chlorhexidine solution, then hydrate the collagen matrix scaffold obtained from a bovine condyle in sterile saline solution for 20 minutes. Next, using surgical soap and a double-edged blade, shave the abdominal skin of an anesthetized six to eight-week-old male Wistar rat, then disinfect the skin three times using a 10%povidone iodine solution.