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Immunology and Infection

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JoVE Journal Immunology and Infection
Het meten van de pH, redoxchemie en degradatieve capaciteit van macropinosomen met behulp van dual-fluorofoor ratiometrische microscopie
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Het meten van de pH, redoxchemie en degradatieve capaciteit van macropinosomen met behulp van dual-fluorofoor ratiometrische microscopie

Article DOI: 10.3791/62733-v • 07:31 min • August 19th, 2021
August 19th, 2021 •
1,2, 2,3
1Department of Biochemistry & Microbiology, Faculty of Science, University of Victoria, 2Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Science, University of Calgary, 3Calvin, Joan and Phoebe Snyder Institute for Chronic Diseases, University of Calgary

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We beschrijven protocollen voor het meten van pH, oxidatieve gebeurtenissen en eiwitvertering in individuele macropinosomen in levende cellen. De nadruk wordt gelegd op dual-fluorofoor ratiometrische microscopie en de voordelen die het biedt ten opzichte van populatiegebaseerde technieken.

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Immunologie en infectie macropinocytose macropinosoom endocytose pH V-ATPase protonpomp reactieve zuurstofsoorten ROS NADPH-oxidase fagocyt macrofaag dendritische cel
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