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Journal / Immunology and Infection / Misurazione del pH, delle sostanze chimiche redox …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Misurazione del pH, delle sostanze chimiche redox e della capacità degradativa dei macropinosomi utilizzando la microscopia a doppio fluoroforo
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Misurazione del pH, delle sostanze chimiche redox e della capacità degradativa dei macropinosomi utilizzando la microscopia a doppio fluoroforo

Article DOI: 10.3791/62733-v • 07:31 min • August 19th, 2021
August 19th, 2021 •
1,2, 2,3
1Department of Biochemistry & Microbiology, Faculty of Science, University of Victoria, 2Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Science, University of Calgary, 3Calvin, Joan and Phoebe Snyder Institute for Chronic Diseases, University of Calgary

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Descriviamo i protocolli per misurare il pH, gli eventi ossidativi e la digestione delle proteine in singoli macropinosomi in cellule vive. L'accento è posto sulla microscopia a doppio fluoroforo ratiometrico e sui vantaggi che offre rispetto alle tecniche basate sulla popolazione.

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Immunologia e Infezione Numero 174 macropinocitosi macropinosoma endocitosi pH V-ATPasi pompa protonica specie reattive dell'ossigeno ROS NADPH ossidasi fagociti macrofagi cellule dendritiche
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