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Microelectrode Array Recording of Sinoatrial Node Firing Rate to Identify Intrinsic Cardiac Pacemaking Defects in Mice
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JoVE Journal Medicine
Microelectrode Array Recording of Sinoatrial Node Firing Rate to Identify Intrinsic Cardiac Pacemaking Defects in Mice

Microelectrode Array Recording of Sinoatrial Node Firing Rate to Identify Intrinsic Cardiac Pacemaking Defects in Mice

DOI:
10.3791/62735-v

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09:20 min

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July 05, 2021

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, , ,
1Department of Biological Sciences,Southern Methodist University

Chapters

  • 00:04Introduction
  • 00:58Dissecting the Sinoatrial Node
  • 03:50Microelectrode Array System Preparation for Recording
  • 04:18Heart Tissue Placement on Microelectrode Array Grid
  • 05:03Data Acquisition
  • 06:10Analysis of Microelectrode Array Recordings for Sinoatrial Node Beat Frequency Measurement
  • 07:13Results: The Sinoatrial Node Beat Frequency Measurement
  • 08:41Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol aims to describe a new methodology to measure intrinsic cardiac firing rate using microelectrode array recording of the whole sinoatrial node tissue to identify pacemaking defects in mice. Pharmacological agents can also be introduced in this method to study their effects on intrinsic pacemaking.

Tags

Keywords: Microelectrode ArraySinoatrial NodeHeart RateCardiac PacemakingMouseDissectionTyrode’s SolutionHeart RemovalAtriumSinoatrial Node Location

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