Name: Three-Dimensional Finger Motion Tracking during Needling: A Solution for the Kinematic Analysis of Acupuncture Manipulation (Video) | JoVE Journal
Uploaded: 2021-10-28T14:00:00.000+00:00
Duration: 8 min 27 s
Description: Learn three-dimensional finger motion tracking for kinematic analysis of acupuncture manipulation using reflective markers. This method enables quantitative evaluation of manual techniques for clinical and educational applications.

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5 minutes

This work was supported by the National Natural Science Foundation of China (Grant Number. 82174506).

This study was approved by the ethics committee of Yueyang Hospital, affiliated with Shanghai University of Traditional Chinese Medicine (reference no. 2021-062), and each participant signed an informed consent form.
1. Experiment preparations
<ol>
	<li>Camera settings:
	<ol>
		<li>Place three tripods in front of the operation table, and connect them with three cameras.</li>
		<li>Set the shooting parameters of the cameras as follows: resolution 1280 x720 pixels, format MP4, full manual mode (M), aperture F1.2, shutter 1/1000s, ISO 6400, automatic white balance, optical zoom 0mm. 
		NOTE: The angle between every two cameras is required to be set at 60&#176;-120&#176; (Figure 1A).</li>
	</ol>
	</li>
	<li>Tracking marker placement:
	<ol>
		<li>Attach seven reflective balls with a diameter of 6.5 mm on the holding-needle hand of each participant for video recording as detailed in steps 1.2.2-1.2.4 and shown in Figure 2A.</li>
		<li>Wrist: Attach one ball on the midpoint of the ulna and radial styloid defined as tracking point &#34;wrist joint &#34;(WJ)</li>
		<li>Thumb: Attach one ball each on the center of thumb nail defined as tracking point &#34;thumb tip&#34; (TT), the IP joint defined as tracking point &#34;thumb end joint&#34; (TEJ), and the metacarpophalangeal (MCP) joint defined as tracking point &#34;thumb base joint&#34; (TBJ), respectively.</li>
		<li>Forefinger: Attach one ball each on the center of the forefinger nail defined as tracking point &#34;forefinger tip&#34; (FT), the proximal interphalangeal (PIP) joint defined as tracking point &#34;forefinger middle joint&#34; (FMJ), and the MCP joint defined as tracking point &#34;forefinger base joint&#34; (FBJ), respectively.</li>
	</ol>
	</li>
</ol>
2. Video shooting and editing
<ol>
	<li>Place a small 15 cm x 15 cm x 15 cm 3D calibration frame with 8 points on the operating table for 3D calibration (Figure 1B,C).</li>
	<li>Remove the frame from the table after taking a video of the calibration frame for at least 8 s.</li>
	<li>Instruct the participants to perform AM on the acupuncture point LI11 (Quchi) of the volunteer, including lifting-thrusting and twirling skills, to control the needle to move up and down and rotate with thumb and forefinger, respectively. Take the videos of the above skills for at least 10 cycles. 
	NOTE: The inclusion and exclusion criteria of the participants to perform AM and volunteers to provide acupuncture points for needling are listed. Participant inclusion: (1) acupuncture teacher or student finished the &#34;lifting-thrusting skill&#34; and &#34;twirling skill&#34; chapter in the course textbook entitled &#39;Acupuncture and Moxibustion Techniques and Manipulations19; (2) participant should have hands-on needling experience with the human body for more than 5 times. Participant exclusion: (1) non-acupuncture teachers or students; (2) acupuncture students without any hands-on needling experience with the human body. Volunteer inclusion: (1) age between 16-60 years old; (2) no obvious skin damage, rupture, suppuration or obvious exudation around LI11 on the right arm. Volunteer exclusion: (1) individuals with a history of smoking, alcohol or drug abuse; (2) individuals with blood system diseases or obvious bleeding tendency; (3) individuals with chronic mental illness or mental disorders; (4) pregnant women; (5) individuals with a history of fainting needles.</li>
	<li>Export all the videos from the cameras to the designated disk of the computer. Rename the 3D calibration videos in cameras 1, 2, 3 as &#34;ca-1.mp4&#34;, &#34;ca-2.mp4&#34; and &#34;ca-3.mp4&#34;.</li>
	<li>Synchronize all manipulation videos in the video editing software (e.g., Adobe premiere pro) and export them named as &#34;lifting-thrusting-1.avi&#34;, &#34;lifting-thrusting-2.avi&#34;, &#34;lifting-thrusting-3.avi&#34;, &#34;twirling-1.avi&#34;, &#34;twirling-2.avi&#34; and &#34;twirling-3.avi&#34;, respectively. 
	&#8203;NOTE: Refer to Supplementary File 1 for the video synchronization instructions of the video editing software used in this study.</li>
</ol>
3. Project configuration of Simi Reality Motion System (motion capture and analysis software)
<ol>
	<li>Open the motion capture and analysis software and choose Create a New Project. Set the project name in Project Label and click Create and Save to save the project in the designated disk.</li>
	<li>Choose Specification &#62; Points &#62; Right/Left Hand and drag the above tracking points from the Predefined Points box into the Used Points box, then click on the Close button to continue. 
	NOTE: All the following steps take the tracking points of the right hand as an example.</li>
	<li>Choose Specification &#62; Connections and click on New Connection
	<ol>
		<li>Input connection name &#34;forefinger III right&#34;. Select &#34;forefinger middle joint right&#34; as the Starting Point and Line to the point &#34;forefinger tip right&#34; in the same window</li>
		<li>Click on the Apply and Close buttons to finish the establishment of the connection.</li>
	</ol>
	</li>
	<li>Add and rename the camera groups
	<ol>
		<li>Right-click on Cameras &#62; Add Camera Group to add new camera groups.</li>
		<li>Right-click on Cameras &#62; Rename to rename the camera groups as &#34;lifting-thrusting camera group&#34; and &#34;twirling camera group&#34;, respectively.</li>
	</ol>
	</li>
	<li>Right-click on the Lifting-Thrusting Camera Group &#62; Add Camera
	<ol>
		<li>Click on the Select File button in the Tracking box.</li>
		<li>Click on Open Existing File and select the operation video &#34;lifting-thrusting-1.avi&#34; in the next window, then click on Apply to finish video import.</li>
		<li>Similar to the above actions, click on Select File in the 3D Calibration box, and import the corresponding calibration video &#34;ca-1.mp4&#34;.</li>
	</ol>
	</li>
	<li>According to step 3.5, continue to import the operation videos &#34;lifting-thrusting-2.avi&#34; and &#34;lifting-thrusting-3.avi&#34;, and their corresponding calibration videos &#34;ca-2.mp4&#34; and &#34;ca-3.mp4&#34; in the Lifting-Thrusting Camera Group, respectively. 
	NOTE: There should be 3 cameras in the Lifting-Thrusting Camera Group in the project window after sections 3.4 and 3.5.</li>
	<li>According to steps 3.4, 3.5, and 3.6, import the twirling skill and calibration videos into the Twirling Camera Group.</li>
</ol>
4. Video analysis
<ol>
	<li>3D calibration for each camera
	<ol>
		<li>Expand Lifting-Thrusting Camera Group and right-click on Lifting-Thrusting-1 &#62; Properties.</li>
		<li>Click on the 3D Calibration button in the 3D Calibration box; input the description and add 8 points by clicking on Add Point button for 8 times</li>
		<li>Click on Apply after setting the name and corresponding X, Y, Z value for each point according to the calibration parameters (Table 1).</li>
		<li>After configuring all points, move the mouse to click each endpoint of the calibration video to finish the 3D calibration.</li>
		<li>Follow steps 4.1.1-4.1.4 to complete the 3D calibration of the other cameras in the same group and the cameras in the Twirling Camera Group.</li>
	</ol>
	</li>
	<li>3D finger motion tracking
	<ol>
		<li>Right-click on Lifting-Thrusting Camera Group &#62; 3D Tracking, select all the cameras, and click on the OK button to open the 3D tracking window.</li>
		<li>Set the Track Using Pattern Matching (all points) for all the cameras and manually click on all the tracking points in the first frame.</li>
		<li>Click on the Search Automatically button to start automatic 3D tracking frame by frame.</li>
		<li>Follow steps 4.2.1-4.2.3 to complete the motion tracking of the Twirling Camera Group. 
		NOTE: If a tracking point is lost during the automatic 3D tracking, select the lost point line, right-click on Discard Point From Here, then re-click the point and the Search Automatically button. Select Yes if the message &#34;No start frame for tracking has been set for 3 selected camera(s). it can be set individually in camera properties. Do you want to set start frame to frame 0 for all cameras without start frame and continue now?&#34; pops up.</li>
	</ol>
	</li>
	<li>Data export
	<ol>
		<li>Right-click on Lifting-Thrusting Camera Group &#62; New 3D Calculation, select all the cameras, and check Update Data Continuously and Store Data Explicitly in File in Create 3D Data window. Click the OK button to continue.</li>
		<li>Right-click on the folder Lifting-Thrusting-3D Coordinates Data &#62; Export, check Column Headings, Tracking Names, Start Time and Frequency, Time Information in First Column, X, Y, Z, v(X), v(Y), v(Z) in the Export window</li>
		<li>Click the Export button to export the data file (*.txt) with the customized name. Export the data file of the Twirling Camera Group in the same way.</li>
	</ol>
	</li>
</ol>
5. Data analysis
NOTE: An original PHP script is used to browse and analyze the data files exported by the motion capture and analysis software. All the source code has been shared in a GitHub repository20.
<ol>
	<li>After the data files exported from the motion capture and analysis software are uploaded to a specific server folder running this script, open the script and input the Username and Password to log in.</li>
	<li>Click on Add New Participant, select the Participant Type and Gender, and input the Participant Name, Age, and Practice Time in the pop-up page; click on Submit to finish adding a new participant.</li>
	<li>Click on Add New Record corresponding to the newly added participant in the list page, then input the Folder Name containing the uploaded data files of the motion capture and analysis software and select the Operation Date; click on Submit to continue.</li>
	<li>Click on Analysis corresponding to the newly added operation record, then select Skill and click on Submit. The script will identify and display all the valid crests and troughs for manual review. 
	NOTE: A certain crest or trough can be reselected manually in the corresponding drop-down list if the script incorrectly identifies it. Based on these crests and troughs, the average values of amplitudes and velocities along three axes of each tracking point and the operating time of lifting, thrusting, twirling left, and twirling right actions can be calculated and displayed by the script. The calculation method of these parameters is shown in Figure 3.</li>
</ol>

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<li>Hunukumbure, A. D., Smith, S. F., Das, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Holistic+feedback+approach+with+video+and+peer+discussion+under+teacher+supervision%5BTitle%5D)+AND+%22BMC+Medical+Education%22%5BJournal%5D)">Holistic feedback approach with video and peer discussion under teacher supervision.</a> BMC Medical Education. 17 (1), 179(2017).</li>
</ol>

The authors have nothing to disclose.

This study established the measurement method of the kinematic parameters of AM in vivo and obtained the data of motion amplitude, velocity, and operation time of the six important tracking points on the thumb and forefinger along three axes. Meanwhile, based on the 3D calibration frame, a 3D stick view and corresponding animation of the thumb and forefinger during needling were generated. The thumb and forefinger movement of AM can be fully displayed with the synchronous playback of kinematic parameter curve an...

As a kind of the clinical skills of traditional Chinese medicine (TCM) and physical stimulation, acupuncture manipulation (AM) is often regarded as an important factor that affects the therapeutic effect of acupuncture1,2. Many studies have confirmed that different AM or different stimulation parameters (needling velocity, amplitude, frequency, etc.) of the same AM resulted in different therapeutic effects3,4,5,6,7. Therefore, the measurement of relevant kinematic parameters of AM and correlation analysis with the therapeutic effect can provide useful data support and reference for the clinical treatment with acupuncture8,9.
The measurement of kinematic parameters of the AM began in the 1980s10. In the early days, the electrical signal conversion technology based on variable resistance was mainly used to convert the displacement signal of the needle body into a voltage or current signal for displaying and recording the amplitude and frequency data of AM11. Moreover, the famous ATP-II Chinese medicine acupuncture technique tester II (ATP-II) with this technology currently has been used by many traditional Chinese medicine universities of China12. After that, with the continuous development and innovation of sensor technology, different types of sensors were used to collect kinematic parameters of AM. For example, the three axes electromagnetic motion sensor was attached to the needle handle to acquire needling amplitude and velocity13; the bioelectric signal sensor was placed on the dorsal horn of the animal&#39;s spinal cord to record needling frequency14, etc. Although the quantitative research of AM based on the above two types of technologies has completed the acquisition of relevant kinematic parameters during needling, its main disadvantages are the inability to perform the real-time non-invasive measurement and the change of operating feel caused by the modification of the needle body.
In recent years, motion tracking technology was gradually applied to the quantitative research of AM15,16. Because it is based on the frame-by-frame analysis of needling video, the measurement of acupuncture parameters can be acquired during in vivo operation without modifying the needle body. This technology has been used to measure the kinematic parameters such as amplitude, velocity, acceleration, and frequency of four tracking points of thumb and forefinger during needling in a two-dimensional (2D) plane and established the corresponding finger stick figure15. Some studies also measured the angle change range of interphalangeal (IP) joint of thumb and forefinger with similar technology9,17,18. However, the current studies on AM analysis are still mainly limited to the 2D motion plane, and the number of tracking points is relatively small. So far, there is no complete three-dimensional (3D) kinematics measurement and analysis method for AM, and no related data was published.
To solve the above problems, this study will use 3D motion tracking technology to measure the kinematic parameters of the seven tracking points of hand during needling. This protocol aims to provide a complete technical solution for the kinematic analysis on AM, as well as the further study on the dose-effect correlation of acupuncture.

<table><tbody><tr><td>3D calibration frame</td><td>Any brand</td><td></td><td>15 x 15 x 15 cm</td></tr><tr><td>Acupuncture needles</td><td>Suzhou Medical Appliance Factory</td><td></td><td>0.35 x 40 mm</td></tr><tr><td>Double-sided tape</td><td>Any brand</td><td></td><td>Round, 1 cm-diameter</td></tr><tr><td>Reflective balls</td><td>Simi Reality Motion Systems GmbH</td><td></td><td>6.5 mm-diameter</td></tr><tr><td>SD card</td><td>Western Digital Corporation</td><td></td><td>SDXC UHS-I</td></tr><tr><td>SD card reader</td><td>UGREEN Group Limited</td><td></td><td>USB 3.0</td></tr><tr><td>Simi Motion</td><td>Simi Reality Motion Systems GmbH</td><td></td><td>Ver.8.5.15</td></tr><tr><td>Swab</td><td>Any brand</td><td></td><td>The volume fraction of ethanol is 70%-80%</td></tr><tr><td>Three cameras</td><td>Victor Company of Japan, Limited</td><td></td><td>JVC GC-PX100BAC</td></tr><tr><td>Three tripods</td><td>Any brand</td><td></td><td></td></tr></tbody></table>

three-dimensional finger motion tracking during needling: a solution for the kinematic analysis of acupuncture manipulation

Three-Dimensional Finger Motion Tracking during Needling: A Solution for the Kinematic Analysis of Acupuncture Manipulation

Three-dimensional (3D) motion tracking has been used in many fields, such as the researches of sport and medical skills. This experiment aimed to use 3D motion tracking technology to measure the kinematic parameters of the joints of fingers during acupuncture manipulation (AM) and establish three technical indicators "amplitude, velocity and time". This method can reflect the operation characteristics of AM and provide quantitative parameters along three axes of multiple finger joints. The current evidence shows that the method has great potential for future applications such as the study of the dose-effect relationship of acupuncture, teaching, and learning of AM, and the measurement and preservation of famous acupuncturists' AM.

After establishing this experimental method, the lifting-thrusting and twirling skills of basic AM of nineteen acupuncture teachers from the School of Acupuncture-Moxibustion and Tuina of Shanghai University of TCM were measured using 3D motion tracking. According to the definition of a joint coordinate system (JCS) for the shoulder, elbow, wrist, and hand proposed by the Standardization and Terminology Committee (STC) of the International Society of Biomechanics21, seven finger tracking points ha...

This experimental method describes a solution for the kinematic analysis of acupuncture manipulation with three-dimensional finger motion tracking technology.

Three-dimensional (3D) motion tracking has been used in many fields, such as the researches of sport and medical skills. This experiment aimed to use 3D ...

three-dimensional-finger-motion-tracking-during-needling-solution-for

Research

JoVE Journal

Medicine

Here, we present a protocol for using the Acupuncture Manipulation Education System (AMES) in the training of acupuncture manipulation skills using phantom acupoints.

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Three-Dimensional Ultrasonic Needle Tip Tracking with a Fiber-Optic Ultrasound Receiver

This protocol designs a cannula that can be used to control the range of motion for the lifting and thrusting manipulation in acupuncture, thereby improving stability and safety. It can thus serve both the clinical application and scientific research of acupuncture treatment.

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Based on the clinical trial, this study provides a standardized operational reference for treating RA with finger joint pain through acupuncture combined with grain-sized moxibustion by stimulating acupoints and warming. It can be used as an effective complementary therapy for RA pain management due to its efficacy and advantages.

Preliminary Study on Acupuncture Combined with Grain-sized Moxibustion for Treating Rheumatoid Arthritis with Finger Joint Pain

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Heterotypic Three-dimensional In Vitro Modeling of Stromal-Epithelial Interactions During Ovarian Cancer Initiation and Progression

We present a simple agarose overlay platform to grow 3D multicellular spheroids using neuroendocrine cancer cell lines. This method provides a very convenient way to examine the effect of therapeutic drugs on the neuroendocrine tumor cells. It could also help us establish human neuroendocrine tumor spheroids for cancer therapy.

Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy

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Three-dimensional Co-culture Model for Tumor-stromal Interaction

Invasion of surrounding normal tissues is a defining characteristic of malignant tumors. We provide here a simple, semi-automated micro-plate assay of invasion into a natural 3D biomatrix that has been exemplified with a number of models of advanced human cancers.

Three-Dimensional (3D) Tumor Spheroid Invasion Assay

Learn three-dimensional finger motion tracking for kinematic analysis of acupuncture manipulation using reflective markers. This method enables quantitative evaluation of manual techniques for clinical and educational applications.

Three-Dimensional Finger Motion Tracking during Needling: A Solution for the Kinematic Analysis of Acupuncture Manipulation

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