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Preparation of High-Temperature Sample Grids for Cryo-EM
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Biochemistry
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JoVE Journal Biochemistry
Preparation of High-Temperature Sample Grids for Cryo-EM

Preparation of High-Temperature Sample Grids for Cryo-EM

DOI:
10.3791/62772-v

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05:05 min

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July 26, 2021

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, ,
1Institute of Biological Chemistry,Academia Sinica, 2Academia Sinica Cryo-EM Facility,Academia Sinica, 3Department of Life Sciences,National Central University, 4Hubei University, 5Institute of Biochemical Sciences,National Taiwan University

Chapters

  • 00:04Introduction
  • 00:46Preparation of the Vitrification Apparatus
  • 01:23Warming Up the Sample and the Tools
  • 01:49Blotting Experiment
  • 02:47Quality Check for the Grids and Data Collection
  • 03:40Results: High-Temperature Sample Grids for Cryo-EM
  • 04:25Conclusion

Summary

Automatic Translation

Automatic Translation

This paper provides a detailed protocol for preparing sample grids at temperatures as high as 70 °C, prior to plunge freezing for cryo-EM experiments.

Tags

Keywords: Cryo-EMHigh-temperature SampleProtein StructureTemperature DependenceVitrificationGrid PreparationSample ApplicationBlottingLiquid EthaneElectron Microscopy

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